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北方园艺  2013 

南瓜SSR-PCR反应体系的优化

, PP. 103-106

Keywords: 南瓜,SSR-PCR,正交实验,体系优化?

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Abstract:

以3个矮生南瓜自交系和2个蔓生南瓜自交系为试材,利用Primer5软件开发南瓜植物中具有特异性的SSR引物,并通过正交实验和梯度PCR试验对影响南瓜SSR-PCR结果的主要条件进行优化筛选,以期建立最佳的SSR-PCR反应体系。结果表明20μL反应体系含有1.5mmol/LMg2+,60ngDNA,0.20mmol/LdNTPs,0.25mmol/LPrimer和1.5UTaq酶,最佳退火温度为44.1℃。?

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