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北方园艺  2014 

山墙藓组织培养条件研究

, PP. 114-117

Keywords: 山墙藓,配子体,消毒方法,培养基,蔗糖浓度

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Abstract:

以山墙藓幼嫩茎尖为外植体,采用L?12(3×24)正交设计方法,对山墙藓配子体消毒方法及培养基类型进行了筛选;通过梯度设计研究不同浓度的蔗糖对配子体形态发生的影响,以建立有效的山墙藓(Tortularuralis)组织培养快繁体系。结果表明山墙藓配子体的最佳消毒方法为浓度3%的次氯酸钠溶液消毒5min后,浓度0.05%的升汞溶液二次消毒20s,Beneck培养基为最适消毒培养基;蔗糖对于山墙藓原丝体的形成和扩展生长均具促进作用,浓度为30g/L时原丝体形成时间最早,扩展直径最大,但抑制了芽体的分化,浓度为15g/L时对芽体分化具明显促进作用。

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