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北方园艺  2012 

柿法呢基焦磷酸合酶基因的克隆与序列分析

, PP. 105-108

Keywords: ,法呢基焦磷酸合酶,克隆,序列分析?

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Abstract:

在萜类生物合成途径中起重要作用的关键酶―法呢基焦磷酸合酶(FarnesylDiphosphateSynthase,FPS),对植物的生长发育、抗逆性以及次生代谢都起着重要的调节作用。通过cDNA末端快速克隆的技术(Rapid-amplificationofcDNAEnds,RACE)从柿果实中克隆到法呢基焦磷酸合酶基因cDNA片段,并进行了序列的相关分析。结果表明所克隆的FPS基因和NCBI数据库中已登录的云杉、腊梅、百合等植物FPS基因核苷酸序列的同源性分别达到88%、75%、73%,并且与香蕉、水稻、橄榄等植物FPS基因氨基酸序列的同源性分别达到72%、67%、66%。该基因已提交GenBank数据库,登录号为JN108022。?

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