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北方园艺  2014 

甜瓜PMR5抗白粉病基因的遗传定位

, PP. 118-122

Keywords: 甜瓜,白粉病,基因,BSA,SSR

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Abstract:

以甜瓜白粉病抗性品种PMR5,感白粉病甜瓜伽师及杂交F?1以及BC?1、BC?2分离群体为试材,接种白粉菌生理小种1,采用抗病遗传分析,并利用BSA(BulkedSegregationAnalysis)结合SSR(SimpleSequenceRepeat)分子标记方法,研究抗白粉病基因的遗传规律获得与其连锁的分子标记,利用获得的分子标记对抗白粉病基因进行遗传定位。结果表明BC?1中抗病与感病分离比为64∶27,BC2中分离比为37∶27,推测PMR5在BC?1中表现2个显性基因,在BC?2中表现单显性基因遗传模式。用BC?2对抗病基因进行遗传定位发现,一共有11个位于LGII的SSR分子标记与抗白粉病基因连锁,抗病基因位于标记CMGA36和SSR25208之间约104113bp。

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