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基于响应面法的二苯并噻吩生物降解条件的优化

Keywords: Plackett-Burman设计,响应面分析法,生物降解,二苯并噻吩,肠杆菌D4

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Abstract:

对脱硫菌D4经菌株的形态特征和16SrDNA序列分析,初步鉴定为肠杆菌属(Enterobactersp.).为提高D4对二苯并噻吩的降解率,在单因素实验的基础上,采用响应面分析法(RSM)对降解条件进行建模优化,筛选出了转速、底物浓度和培养温度3个影响显著的因素.确定了优化后的降解条件:转速175r/min,二苯并噻吩浓度0.1mmol/L,培养温度30℃.优化后的降解率为59.83%,比优化前提高了近10%.

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