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胡桃醌含药血清体外抗肿瘤实验研究

Keywords: 胡桃醌,血清药理学,药动学,A549细胞,细胞周期

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Abstract:

研究胡桃醌(Juglone)含药血清对人肺癌A549细胞的生长抑制作用及对细胞周期的影响.以血清药理学原理为基础,通过MTT法检测胡桃醌对A549细胞的生长抑制作用,并筛选最佳药物浓度;利用流式细胞仪分析胡桃醌对A549细胞周期的影响,并用Westernblot验证.实验结果表明,胡桃醌的半衰期T1/2=136min,MTT法显示其对体外培养的人肺癌A549细胞具有明显抑制作用,比较12,24,48h细胞存活率得出10%高剂量组(0.107μg/mL)胡桃醌血清抑制作用较好,同时,胡桃醌可通过凋亡途径阻滞细胞于S期,增加caspase-3的表达.胡桃醌对A549细胞可能具有生长抑制作用,将细胞阻滞在S期,诱导细胞凋亡,上调caspase-3表达量.

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