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基于分子印迹膜的表面等离子体共振传感器检测黄体酮

DOI: 10.15918/j.tbit1001-0645.2015.02.019

Keywords: 分子印迹膜 表面等离子体共振传感器 黄体酮

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Abstract:

分别采用热聚合和光聚合的方法在金膜表面合成黄体酮分子印迹膜,并将其作为识别元件应用于表面等离子体共振传感器检测黄体酮. 通过对其进行洗脱及吸附研究发现:采用乙腈/乙酸混合液可以快速将黄体酮洗脱下来,洗脱前后共振角分别移动了1.15°和0.72°;该传感器实现对黄体酮的最低检测限均为10-14 g/mL;在10-14~10-6 g/mL的浓度范围内,共振角度的变化与黄体酮浓度的负对数成线性关系. 同时将具有黄体酮识别位点的分子印迹膜分别吸附浓度为10-4 g/mL的雌二醇,睾酮和睾酮丙酸酯溶液,结果显示均没有非特异性吸附作用. 以上结果表明,基于黄体酮分子印迹膜的表面等离子体共振传感器能够对黄体酮实现快速定量的专一性检测

References

[1]  Zheng C, Huang Y P, Liu Z S. Synthesis and theoretical study of molecularly imprinted monoliths for HPLC[J]. Analytical and Bioanalytical Chemistry, 2013,405(7):2147-2161.
[2]  Homola J. Surface plasmon resonance sensors for detection of chemical and biological species[J]. Chemical Reviews, 2008,108(2):462-493.
[3]  Mayer K M, Hafner J H. Localized surface plasmon resonance sensors[J]. Chemical Reviews, 2011,111(6):3828-3857.
[4]  Ricanyova J, Gadzala-Kopciuch R, Reiffova K, et al. Molecularly imprinted adsorbents for preconcentration and isolation of progesterone and testosterone by solid phase extraction combined with HPLC[J]. Adsorption-Journal of the International Adsorption Society, 2010,16 (4-5):473-483.
[5]  Guil-Luna S, Stenvang J, Brunner N, et al. Detction of progesterone receptor by QPCR analysis from formalin-fixed paraffin wax-embedded canine mammary tissues [J]. Journal of Comparative Pathology, 2013,148 (1):61-61.
[6]  Carmon K S, Baltus R E, Luck L A. A biosensor for estrogenic substances using the quartz crystal microbalance[J]. Analytical Biochemistry, 2005,345(2):277-283.
[7]  Kadioglu Y, Albayrak M, Demirkaya F. Development and validation of RP-HPLC-DAD method for determination of progesterone in capsule[J]. Reviews in Analytical Chemistry, 2010, 29 (3-4): 197-210.
[8]  Fahlbusch F B, Ruebner M, Rascher W, et al. Combined quantification of corticotropin-releasing hormone, cortisol-to-cortisone ratio and progesterone by liquid chromatography-tandem mass spectrometry in placental tissue[J]. Steroids, 2013,78(9):888-895.
[9]  Ye F, Liu J, Huang Y, et al. Competitive immunoassay of progesterone by microchip electrophoresis with chemiluminescence detection[J]. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 2013,936:74-79.
[10]  Wu Y, Mitchell J, Cook C, et al. Evaluation of progesterone-ovalbumin conjugates with different length linkers in enzyme-linked immunosorbant assay and surface plasmon resonance-based immunoassay[J]. Steroids, 2002,67(7):565-572.
[11]  Gillis E H, Traynor I, Gosling J P, et al. Improvements to a surface plasmon resonance-based immunoassay for the steroid hormone progesterone[J]. Journal of AOAC International, 2006,89(3):838-842.
[12]  Shimizu K D, Stephenson C J. Molecularly imprinted polymer sensor arrays [J]. Current Opinion in Cell Biology, 2010,14 (6):743-750.
[13]  Whitcombe M J, Chianella I, Larcombe L, et al. The rational development of molecularly imprinted polymer-based sensors for protein detection[J]. Chemical Society Reviews, 2011,40(3):1547-1571.
[14]  Lepinay S, Kham K, Millot M C, et al. In-situ polymerized molecularly imprinted polymeric thin films used as sensing layers in surface plasmon resonance sensors: mini-review focused on 2010-2011[J]. Chemical Papers, 2012,66 (5):340-351.
[15]  Ansell R J. Molecularly imprinted polymers for the enantioseparation of chiral drugs[J]. Advanced Drug Delivery Reviews, 2005,57(12):1809-1835.
[16]  Li J P, Li Y P, Wei X P. Molecularly imprinted electrochemical sensors based on the double-amplification from the electro-catalysis of poly-melamine and the enzymatic catalysis of HRP for chlortoluron determination[J]. Acta Chimica Sinica, 2012,70(17):1853-1857.

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