OALib Journal期刊
pH区带逆流色谱法分离纯化马钱子中马钱子碱和士的宁
, PP. 1703-1707
Keywords: pH区带逆流色谱法 ,马钱子 ,马钱子碱 ,士的宁
Abstract:
建立了pH区带逆流色谱分离制备马钱子中生物碱类成分马钱子碱和士的宁的方法。溶剂系统为V(甲基叔丁基醚);V(乙腈);V(水)=2;2;3,静置分层后,上相加入三乙胺,使其浓度为10mmol/L,作为固定相;下相加入HCl,使其浓度为10mmol/L,作为流动相。从308mg马钱子总生物碱中分离得到50mg马钱子碱和120mg士的宁,得率分别为72.8%和85.1%;纯度分别为96.8%和98.3%。并采用LC-ESI-MS和13CNMR对目标化合物的结构进行了鉴定。
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