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OALib Journal期刊
ISSN: 2333-9721
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基于碳纳米管的脂质体电化学发光免疫传感器检测人免疫球蛋白G
, PP. 1329-1332
Keywords: 电化学发光,碳纳米管,脂质体,免疫传感器,人免疫球蛋白G
Abstract:
将人免疫球蛋白G(hIgG)抗体固定在多壁碳纳米管修饰的玻碳电极表面,制备了一种电化学发光(ECL)免疫传感器。以hIgG抗体标记的联吡啶钌脂质体为标记物,采用三明治型检测方式,成功建立了hIgG的ECL免疫检测技术。电化学发光强度与hIgG的浓度在0.01～0.8μg/L范围内呈良好的线性关系;线性回归方程为y=618.7x(μg/L)-23.9(n=6,r=0.995);检出限为0.004μg/L。用于人血清中hIgG的检测,结果令人满意。
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