OALib Journal期刊
液相色谱-串联质谱法测定鸡组织中沃尼妙林残留
, PP. 1031-1035
Keywords: 沃尼妙林 ,鸡组织 ,残留 ,液相色谱-串联质谱
Abstract:
建立了鸡组织中沃尼妙林残留的液相色谱-电喷雾串联质谱(LC-ESI-MS/MS)检测方法。样品用乙腈提取后,过Strata-X-C固相萃取小柱净化。以乙腈-0.1%甲酸水溶液(35:65,V/V)为流动相,经LunaC18色谱柱分离,采用电喷雾电离,多反应监测(MRM)正离子模式对沃尼妙林进行定性与定量分析。采用基质匹配法,对鸡皮肤、肌肉、肾脏和肝脏中沃尼妙林的含量进行标准校正,在5(7.5)～500μg/kg范围内线性良好(r>0.998),在鸡肝脏中的检出限(LOD)及定量限(LOQ)分别为4.0和7.5μg/kg;其它3种组织中的LOD及LOQ分别为2.5和5.0μg/kg。在5(7.5),50及500μg/kg添加水平下,4中组织中沃尼妙林的平均回收率为78.5%～104.0%。日内相对标准偏差(RSD)为1.2%～9.8%,日间相对标准偏差为3.7%～13.2%。
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