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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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基于双分子识别荧光猝灭法高选择性测定多巴胺
, PP. 1711-1715
Keywords: 双分子识别,荧光猝灭法,CdTe,量子点,多巴胺
Abstract:
制备了以二硫基琥珀酰亚胺丙酸酯,4-巯基苯硼酸功能化CdTe量子点探针,对其进行了X-射线光电子能谱和透射电镜表征。在431nm激发波长下,对探针及加入多巴胺后进行发射光谱扫描,最大发射峰红移,在最大发射波长处,多巴胺对合成的探针具有猝灭效应,且多巴胺对体系的猝灭程度与多巴胺的量呈良好的线性关系,据此建立了一种直接、灵敏、选择性好的测定多巴胺的方法。在最佳条件下,多巴胺的其线性范围为0.02~20.0mmol/L,线性回归方程为ΔF=20.1+31.7C(mmol/L),相关系数为R2=0.987,检出限为4.9nmol/L。本方法已成功用于多巴胺注射液、血清及尿样中多巴胺的测定,结果满意。
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