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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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弱阴离子超高交联树脂固相萃取-高效液相色谱-紫外法测定人尿中高香草酸的含量
DOI: 10.3724/SP.J.1096.2012.11446, PP. 1175-1180
Keywords: 固相萃取,弱阴离子超高交联树脂,高效液相色谱法,尿液,高香草酸
Abstract:
建立了人体尿液中高香草酸含量的固相萃取-高效液相色谱-紫外测定方法。合成了弱阴离子超高交联树脂固相萃取填料,并对其进行吸附性能考察。利用此萃取剂对尿液中的高香草酸进行选择性吸附,洗脱液富集后以甲醇-乙酸(15:85,V/V)溶液为流动相,C18为固定相,于280nm测定其中的高香草酸。方法验证结果表明,高香草酸在2.26-145mg/L浓度范围内呈良好的线性关系;检出限为0.45mg/L;方法的平均加标回收率大于90%;RSD小于4.2%。采用本方法对8名健康人尿样中的高香草酸含量进行了测定,结果为1.55-6.79mg/L。表明本方法选择性好、灵敏度高、准确可靠。
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