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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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肽段化学衍生技术及其在质谱高灵敏度鉴定中的应用进展
DOI: 10.3724/SP.J.1096.2012.11452, PP. 1123-1129
Keywords: 化学衍生,高灵敏检测,质谱,多肽,综述
Abstract:
鸟枪法串联质谱蛋白质鉴定是蛋白质组学研究中广泛采用的策略。然而,蛋白质组样品的高复杂性、宽动态范围分布使得低丰度肽段以及难于离子化肽段的质谱检测仍然存在着巨大的挑战。为了提高质谱检测灵敏度,通过化学衍生技术在多肽中引入易于离子化的小分子标签的方法获得了广泛关注。本文综述了近年来应用于多肽及其翻译后修饰的化学衍生试剂,侧重其在高灵敏度质谱分析中的应用进展,并展望了化学衍生技术的发展方向及其在蛋白质组学中的应用前景。
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