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OALib Journal期刊
ISSN: 2333-9721
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电化学研究铝及其纳米Al13对谷胱甘肽还原酶活性的影响
DOI: 10.3724/SP.J.1096.2012.10618, PP. 584-588
Keywords: 纳米Al13,谷胱甘肽还原酶,还原型辅酶Ⅱ,多壁碳纳米管,修饰电极
Abstract:
采用滴涂法制备了碳纳米管(MWNTs)/壳聚糖(CHIT)修饰玻碳电极(GCE),较为明显的使还原型辅酶Ⅱ(NADPH)在裸GCE上的氧化电势从700mV降低到修饰电极上的380mV,并且明显增大了催化电流。氧化电流与NADPH浓度在0.08~2.60mmol/L范围内呈线性关系;检出限为18.3μmol/L。利用此修饰电极,采用计时电流i-t法,在谷胱甘肽还原酶(GR)催化"氧化型谷胱甘肽+NADPH"生成"还原型谷胱甘肽+NADP+"反应中,通过检测NADPH在电极上催化电流的变化情况,分析了不同酸度和浓度下的Al3+和nano-Al13的加入对GR酶促反应初速率V0、米氏常数Km及反应最大速率Vmax的影响。
| [1] | 13 Musameh M, Wang J, Merkoci A, Lin Y. Electrochem. Commun., 2002, 4(10): 743~746
|
| [2] | 14 Yan J, Zhou H, Yu P, Su L, Mao L. Adv. Mater., 2008, 20(15): 2899~2906
|
| [3] | 15 Du P, Wu P, Cai C. J. Electroanal. Chem., 2008, 624(1-2): 21~26
|
| [4] | 16 Cai L, Xie Y, Li L, Li H, Yang X, Liu S. Colloids Surf. B, 2010, 81(1): 123~129
|
| [5] | 17 Luo H, Shi Z, Li N, Gu Z, Zhuang Q. Anal. Chem., 2001, 73(5): 915~920
|
| [6] | 18 Guibaud G , Gauthier C.J. Inorg. Biochem., 2005, 99(9): 1817 ~1821
|
| [7] | 19 Louzada P R, Sebollela A, Scaramello M E, Ferreira S T. Biophys. J., 2003, 85(6): 3255~3261
|
| [8] | 1 Ma J F, Ryan P R, Delhaize E. Trends. Plant Sci., 2001, 6(6): 273~278
|
| [9] | 2 Orihuela D, Meichtry V, Pizarro M. J. Inorg. Biochem., 2005, 99(9): 1879~1886
|
| [10] | 3 Andrei C M, Adrian I O, Mircea M. Journal of Alzheimer''s Disease, 2004, 6(3): 315~328
|
| [11] | 4 Gilbert N. Nature, 2009, 460(7258): 937~940
|
| [12] | 5 Wang N, Huang D, Zhang J, Cheng J, Yu T, Zhang H, Bi S. J. Phys. Chem. C., 2008, 112(46): 18034~18038
|
| [13] | 6 Boscolo P R S, Menossi M, Jorge R A. Phytochemistry, 2003, 62(2): 181~189
|
| [14] | 7 Sarma G N, Savvides S N, Becker K, Schirmer R H, Karplus P A. J. Mol. Biol., 2003, 328(4): 893~907
|
| [15] | 8 Cardoso L A, Ferreir S T, Lima M H. Comp. Biochem. Phy. A, 2008, 151(3): 313~321
|
| [16] | 9 Nagalakshmi N, Prasad M N V. Plant Sci., 2001, 160(2): 291~299
|
| [17] | 10 Fanjul-Bolado P, Queipo P, Lamas-Ardisana P J, Costa-García A. Talanta, 2007, 74(1): 427~433
|
| [18] | 11 YE She-Fang, WEN Wen, WANG Yi-Fang, LIN Cui-Lin, Wu Yi-Hui, ZHANG Qi-Qing. Chem. J. Chinese Universities, 2010,31(3): 497~501
|
| [19] | 干 宁, 罗乃兴, 李天华, 郑 磊, 倪敏君. 分析化学,2010,38(11): 1556~1562
|
| [20] | 叶社房, 温 雯, 王义芳, 林翠林, 吴艺晖, 张其清. 高等学校化学学报, 2010,31(3): 497~501
|
| [21] | 12 GAN Ning, LUO Nai-Xing, LI Tian-Hua, ZHENG Lei, NI Min-Jun. Chinese J. Anal. Chem., 2010, 38(11): 1556~1562
|
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