OALib Journal期刊
基于噬菌体展示抗体的电化学发光免疫传感器检测相思子毒素研究
DOI: 10.3724/SP.J.1096.2013.30084
Keywords: 噬菌体展示抗体 ,相思子毒素 ,电化学发光 ,免疫传感器
Abstract:
以多克隆抗体包被磁性微球制备捕获探针,以表面负载大量三联吡啶钌标记物的噬菌体展示抗体为发光探针有效放大信号,成功建立了相思子毒素电化学发光免疫传感检测方法。利用本方法检测相思子毒素,浓度在0.005~100μg/L范围内与电化学发光强度呈良好的对数线性关系,拟合方程为lgY=0.701lgX+1.767(R=0.9964,N=7,p<0.0001),检测限达0.005μg/L(S/N=3)。与采用单克隆抗体制备标记探针相比,检测灵敏度提高20倍,可用于相思子毒素的痕量检测。
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