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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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超声萃取-同位素稀释液相色谱-质谱法检测血清中氢化可的松
DOI: 10.3724/SP.J.1096.2013.30215, PP. 1439-1443
Keywords: 氢化可的松,同位素稀释,液相色谱-质谱,超声萃取,血清
Abstract:
建立了液相色谱-质谱检测血清中氢化可的松含量的方法。血清加内标后平衡30min,加乙腈除蛋白,将混合液超声10min,再以3000r/min离心15min,取出上清液用氮气吹干,用水复溶后,以相同体积乙酸乙酯萃取两次,将两次萃取的乙酸乙酯合并,氮气吹干,然后用200μL流动相复溶,液相分离采用ZorbaxEclipsePlusC18色谱柱,流动相为甲醇和含0.1%甲酸的水溶液(43:57,V/V),日间和日内标准偏差分别为0.3%-0.7%和0.3%-0.6%,回收率为99.6%-101.0%,定量限为1.68μg/kg。运用本方法检测了3种人体血清中氢化可的松含量,并研究了血清中氢化可的松的稳定性。
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