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OALib Journal期刊
ISSN: 2333-9721
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谷胱甘肽修饰的金纳米粒子探针比色法检测神经元素3
DOI: 10.11895/j.issn.0253-3820.140088, PP. 955-961
Keywords: 神经元素3,多肽,金纳米粒子,比色
Abstract:
采用柠檬酸钠还原法合成了粒径为13nm的金纳米粒子，并在其表面修饰上谷胱甘肽（GSH-AuNPs）。在一定的盐浓度范围内，谷胱甘肽能保护金纳米粒子免受盐诱导的聚集。当神经元素3（Neurogenin3，ngn3）多肽片段存在时，在一定的盐浓度下，ngn3片段能够诱导GSH-AuNPs发生聚集，使金纳米粒子溶液由红色变成蓝色。以谷胱甘肽修饰的金纳米粒子为探针，建立了快速检测ngn3片段的比色方法。通过优化得到的最适实验条件为：ngn3与GSH-AuNPs的平衡反应时间10min，缓冲液pH=6.0，NaCl浓度100mmol/L。在优化条件下，检测ngn3的线性范围为20~300μg/L，检出限（LOD）为8μg/L。结果表明，本方法具有良好的选择性，可用于实际样品的检测。
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