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分析化学  2015 

在线固相萃取-高效液相色谱法检测食用油中15+1种欧盟优控多环芳烃

DOI: 10.11895/j.issn.0253-3820.150300, PP. 1743-1748

Keywords: 在线固相萃取,多环芳烃,高效液相色谱,食用油

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Abstract:

建立了植物油中15+1种欧盟优控多环芳烃的在线固相萃取-高效液相色谱-紫外/荧光(online-SPE-HPLC-UV/FLD)检测分析方法。食用油样品经异丙醇稀释定容并过滤,固相萃取柱ChromSpherPi(80mm×3mm)在线净化,反相C18PAH专用柱(250mm×4.6mmi.d,5μm)分离,异丙醇、乙腈和水为流动相,梯度洗脱,紫外(220nm)和荧光检测器检测。结果表明,15+1种多环芳烃在相应的线性范围内峰面积与质量浓度之间线性良好(R2>0.99);检出限为0.03~12.23μg/kg;3个水平加标回收率为65.3%~110.5%;相对标准偏差为0.1%~9.8%。

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