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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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基于λ核酸外切酶辅助放大的化学发光传感器用于DNA的灵敏检测
DOI: 10.11895/j.issn.0253-3820.150453, PP. 1513-1519
Keywords: λ核酸外切酶,G-四链体/血红素DNA酶,化学发光,DNA
Abstract:
基于G-四链体/血红素DNA酶的高催化活性以及λ核酸外切酶(λexo)的辅助放大功能,以17个碱基的DNA片段作为模型分析物,构建了一种快速、灵敏、特异性好的新型Luminol-H2O2-G-四链体/血红素DNA酶化学发光传感体系。考察了λexo用量、Luminol浓度、H2O2浓度、溶液pH值对体系化学发光强度的影响。在最佳实验条件下,即:pH=9.0,10unitsλexo,1.0×10-3mol/LLuminol,3.0×10-2mol/LH2O2,测得DNA在2.0×10-12-8.0×10-9mol/L的浓度范围内与Luminol的相对化学发光强度之间呈现良好的线性关系,检出限(3σ)为0.7×10-13mol/L。本方法可以区分不同错配的核酸序列,并可用于复杂生物样品的分析。
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