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分析化学 2015

硒-汞双元素标记策略识别硒蛋白/多肽

DOI: 10.11895/j.issn.0253-3820.150327, PP. 1265-1271

徐明,杨利民,王秋泉

Keywords: 硒,汞,硒蛋白/多肽,电感耦合等离子体质谱

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Abstract:

提出并发展了一种基于电感耦合等离子体质谱(ICP-MS)的双元素标签标记策略来选择性识别和检测硒蛋白/多肽,其中内源元素硒(Se)作为硒蛋白/多肽分子的识别元素,外源元素汞(Hg)作为硒蛋白/多肽和含硒蛋白/多肽分子的区分元素。通过对硒代半胱氨酸(SeCys)和谷胱甘肽过氧化酶1(GPx1)两种模型分子的研究,外源邻羧基苯硫甲基汞(CH3Hg-THI)动态解离的CH3Hg+能够选择性标记硒代半胱氨酸残基(SeCys)中硒醇基(-SeH),但不能标记含硒蛋白/多肽分子的硒代蛋氨酸残基(SeMet)中的—SeCH3,进而依据Se和Hg的ICP-MS信号识别和检测硒蛋白/多肽。本方法应用于富硒酵母水溶性提取液的分析,结果表明,提取液中的硒蛋白/多肽能够被有效识别和检测,验证了Se-Hg双元素标签标记策略的发展是ICP-MS识别和检测硒蛋白/多肽的一种可行且优越的途径。

References

[1]	1 Kjeldahl J Z. Anal. Chem., 1883, 22(1): 366-382
[2]	2 Cvetkovic A, Menon A L, Thorgersen M P, Scott J W, Poole F L, Jenney F E, Lancaster W A, Praissman J L, Shanmukh S, Vaccaro B J, Shanmukh S, Vaccaro B J, Trauger S A, Kalisiak E, Apon J V, Siuzdak G, Yannone S M, Tainer J A, Adams M W. Nature, 2010, 466(7307): 779-782
[3]	3 XU Ming, YAN Xiao-Wen, YANG Li-Min, WANG Qiu-Quan. Scientia Sininca Chimica, 2011, 41(4): 663-677 徐明, 严晓文, 杨利民, 王秋泉. 中国科学: 化学, 2011, 41(4): 663-677
[4]	4 Yan X W, Yang L M, Wang Q Q. Anal. Bioanal. Chem., 2013, 405(17): 5663-5670
[5]	5 WANG Qiu-Quan,TANG Nan-Nan, YANG Li-Min. Scientia Sininca Chimica, 2014, 44(5): 664-671 王秋泉, 唐南南, 杨利民. 中国科学: 化学, 2014, 44(5): 664-671
[6]	6 Zhang C, Zhang Z, Yu B, Shi J, Zhang X. Anal. Chem., 2002, 74(1): 96-99
[7]	7 Baranov V I, Quinn Z, Bandura D R, Tanner S D. Anal. Chem., 2002, 74(7): 1629-1636
[8]	8 Sanz-Medel A, Montes-Bayon M, Fernandez Sanchez M L. Anal. Bioanal. Chem., 2003, 377(2): 236-247
[9]	9 Szpunar J. Analyst, 2005, 130(4): 442-465
[10]	10 Prange A, Prfrock D. J. Anal. At. Spectrom., 2008, 23(4): 432-459
[11]	11 Bettmer J, Montes Bayón M, Encinar J R, Fernández Sánchez M L, Fernández de la Campa Mdel R, Sanz Medel A. J. Proteomics, 2009, 72(6): 989-1005
[12]	12 Mounicou S, Szpunar J, Lobinski R. Chem. Soc. Rev., 2009, 38(4): 1119-1138
[13]	13 Wang M,Feng W, Zhao Y, Chai Z. Mass Spectrom. Rev., 2010, 29(2): 326-348
[14]	14 Xu M, Yang LM, Wang Q Q. J. Anal. At. Spectrom., 2008, 23(11): 1545-1549
[15]	15 Guo Y F, Chen L Q, Yang L M, Wang Q Q. J. Am. Soc. Mass Spectrom., 2008, 19(8): 1108-1113
[16]	16 Guo Y F, Xu M, Yang L M, Wang Q Q. J. Anal. At. Spectrom., 2009, 24(9): 1184-1187
[17]	17 Xu M, Yan X W, Xie Q Q. Yang L M, Wang Q Q. Anal. Chem., 2010, 82(5): 1616-1620
[18]	18 Xu M, Yang L M, Wang Q Q. Chem. Eur. J., 2012, 18(44): 13989-13993
[19]	19 Xu M, Yang L M, Wang Q Q. Metallomics, 2013, 5(7): 855-860
[20]	20 Mounicou S, Shah M, Meija J, Caruso J A, Vonderheide A P, Shann J. J. Anal. At. Spectrom., 2006, 21(4): 404-412
[21]	21 Li Y F, Chen C Y, Li B, Wang Q, Wang J X, Gao Y X, Zhao Y L, Chai Z F. J. Anal. At. Spectrom., 2007, 22(8): 925-930
[22]	22 Li Y F, Dong Z Q, Chen C Y, Li B, Gao Y X, Qu L Y, Wang T C, Fu X, Zhao Y L, Chai Z F. Environ. Sci. Technol., 2012, 46(20): 11313-11318
[23]	23 Behne D, Kyriakopoulos A. Annu. Rev. Nutr., 2001, 21(1): 453-473
[24]	24 Huber R E, Criddle R S. Arch. Biochem. Biophys., 1967, 122(1): 164-173
[25]	25 Arnér E S. Exp. Cell Res., 2010, 316(8): 1296-1303
[26]	26 Margis R, Dunand C, Teixeira F K, Margis-Pinheiro M. FEBS J., 2008, 275(15): 3959-3970
[27]	29 Mester Z, Willie S, Yang L, Sturgeon R, Caruso J A, Fernández M L, Fodor P, Goldschmidt R J, Goenaga-Infante H, Lobinski R, Maxwell P, McSheehy S, Polatajko A, Sadi B B, Sanz-Medel A, Scriver C, Szpunar J, Wahlen R, Wolf W. Anal. Bioanal. Chem., 2006, 385(1): 168-180
[28]	30 Rao Y, McCooeye M, Windust A, Bramanti E, D''Ulivo A, Mester Z. Anal. Chem., 2010, 82(19): 8121-8130
[29]	27 Nakamura W, Hosoda S, Hayashi K. Biochim. Biophys. Acta, 1974, 358(1): 251-261
[30]	28 Lobanov A V, Hatfield D L, Gladyshev V N. Biochim. Biophys. Acta, 2009, 1790(11): 1424-1428
[31]	31 Bierla K, Szpunar J, Yiannikouris A, Lobinski R. Trends Anal. Chem., 2012, 41: 122-132
[32]	32 Bierla K, Bianga J, Ouerdane L, Szpunar J, Yiannikouris A, Lobinski R. J. Proteomics, 2013, 87: 26-39
[33]	33 Li H M, Luo Y C, Li Z X, Yang L M, Wang Q Q. Anal. Chem., 2012, 84(6): 2974-2981

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