全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
电化学  2011 

石墨烯修饰玻碳电极用于急性早幼粒细胞白血病PML/RARa融合基因的检测

, PP. 195-198

Keywords: 石墨烯,电化学DNA传感器,PML/RAR&alpha,融合基因

Full-Text   Cite this paper   Add to My Lib

Abstract:

应利用电化学还原法将固定在玻碳电极表面的氧化石墨还原为石墨烯,然后再利用偶联活化剂将氨基修饰的急性早幼粒细胞白血病(APL)PML/RARα融合基因序列探针固定到石墨烯修饰电极表面,以亚甲基蓝(MB)为电化学杂交指示剂,并由差分脉冲伏安法检测人工合成APL的PML/RARα融合基因.结果表明,石墨烯对MB的检测信号起到了很好的增敏作用,杂交前后MB还原峰电流差值与靶标链DNA浓度在5×10-10~2.5×10-9mol/L范围内呈线性关系,检出限为8×10-11mol/L.该方法简单、特异性好,有望用于实际样品的检测.

References

[1]  GeimA K,Novoselov K S.The rise of graphene[J]. Nature Mater,2007, 6(3): 1 183 - 191.
[2]  Shan C S, Yang H F, Han D X, et al. Water-Soluble Graphene Covalently Functionalized by Biocompatible Poly-L-lysine [J]. Langmuir, 2009, 25(20) :12030-12033.
[3]  Fu C L, Yang W S, Chen X, et al.Direct electrochemistry of glucose oxidase on a graphite nanosheet-Nafion composite film modified electrode[J]. Electrochem Commun, 2009, 11(5):997-1000.
[4]  Zhang J Y(张建瑜),Tang J M (唐加明). Clinic study about 21 cases of acute promyelocytic leukemia [J]. International Medicine & Health Guidance News (国际医药卫生导报), 2007, 13(12): 28-31.
[5]  Yoo S J, Seo E J, Lee J H, et al. A complex, four-way variant t(15; 17) in acute promyelocytic leukemia[J]. Cancer Genet Cytogenet, 2006, 167 (2): 168-171.
[6]  Han J Y, Kim K E, Kim K H, et al. Identification of PML-RARA rearrangementbyRT-PCR and sequencing in an acute promyelocytic leukemiawithout t(15; 17)on G-banding and FISH[ J]. Leukemia Research, 2007, 31(2): 239-243.
[7]  Wei N, Chen J H, Zhang J, et al. An electrochemical biosensor for detection of PML/RARA fusion gene using capture probe covalently immobilized onto poly-calcon carboxylic acid modified glassy carbon electrode [J]. Talanta, 2009, 78 (4-5): 1227-1234.
[8]  Guo H L, Wang X F, Qian Q Y, et al. A Green approach to the synthesis of graphene nanosheets[J]. ACS Nano, 2009, 3 (9) : 2653-2659.
[9]  Hummers W S, Offeman R E. Preparation of graphite oxide [J]. J Am Chem Soc, 1958, 80(6):1339-1339.
[10]  Nethravathi C, Viswanath B, Shivakumara C, et al. The production of semectite clay/graphene composites through delamination and co-stacking[J]. Carbon, 2008, 46(13): 1773-1781.
[11]  Erdem, A, Kerman K, Meric B, et al. Methylene blue as a novel electrochemical hybridization indicator[J]. Electroanalysis, 2001, 13(3):219-223.
[12]  Lin X H, Wu P, Chen W, et al. Electrochemical DNA biosensor for the detection of short DNA species of chronic myelogenous leukemia by using methylene blue [J]. Talanta, 2007, 72 (3): 468-471.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133