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电化学  2014 

表面分子印迹磁性纳米粒子的制备及其血红蛋白传感应用

DOI: 10.13208/j.electrochem.140437, PP. 521-526

Keywords: 分子印迹聚合物,磁性纳米粒子,多巴胺,血红蛋白,电化学传感器

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Abstract:

以Fe3O4磁性纳米粒子为载体、多巴胺(DA)为功能单体、血红蛋白(Hb)为模板分子,用氯铂酸氧化DA生成聚多巴胺(PDA),同时氯铂酸还原为铂纳米粒子(PtNPs),与Hb一起负载于Fe3O4纳米粒子表面,洗脱Hb后合成了表面分子印迹磁性纳米粒子(印迹Fe3O4/PDA-PtNPs).将印迹Fe3O4/PDA-PtNPs修饰于磁性玻碳基底表面,制得印迹Fe3O4/PDA-PtNPs修饰电极.实验结果表明,印迹Fe3O4/PDA-PtNPs具有良好的水溶性,粒径分布均匀,生成的PtNPs具有良好的导电性和刚性.用印迹Fe3O4/PDA-PtNPs构建的传感器对Hb具有良好的识别性,在0.14~2.7μg·mL-1Hb浓度范围与交流阻抗变化值呈良好的线性关系,检出限(S/N=3)为0.05μg·mL-1.

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