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电化学  2014 

孔蛋白-磷脂仿生膜的葡萄糖氧化酶电化学

DOI: 10.13208/j.electrochem.131176, PP. 439-443

Keywords: 孔蛋白,仿生膜,葡萄糖氧化酶,电化学,双肉豆蔻磷脂酰胆碱

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Abstract:

采用孔蛋白(MspA)和双肉豆蔻磷脂酰胆碱(DMPC)在玻碳(GC)基底表面成功构建有仿生特性的纳米通道膜,同时将葡萄糖氧化酶(GOD)修饰于膜上.使用循环伏安法研究GOD/MspA-DMPC/GC电极的GOD直接电化学过程以及其对氧气和葡萄糖的响应.研究发现,MspA与DMPC形成的仿生纳米通道膜内,GOD在接近生物体系FAD/FADH标准电位处实现了自身两质子、两电子表面控制的电化学反应.MspA与DMPC的仿生纳米通道膜体系为GOD提供了理想活性环境.

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