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电化学  2014 

凹土-辣根过氧化物酶复合材料制备及其细胞外过氧化氢检测

DOI: 10.13208/j.electrochem.130883, PP. 260-265

Keywords: 辣根过氧化物酶,凹土,过氧化氢,巨噬细胞

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Abstract:

将辣根过氧化物酶(HRP)固定到凹凸棒石粘土(以下简称凹土,Attapulgite)表面,制得了HRP-凹土纳米复合物(HRP-Attapulgite),采用电化学阻抗、紫外光谱和红外光谱技术表征了HRP固定化过程.HRP-Attapulgite电化学性质测试表明,凹土能促进HRP的直接电子转移,其循环伏安曲线有一对良好的氧化还原峰,峰电位分别为Epc=-370mV,Epa=-300mV,式量电位E0′=-335mV;凹土表面HRP的H2O2响应电流与浓度(0.3~75μmol·L-1)呈线性关系.该电极还可用于巨噬细胞中微量H2O2的测定.

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