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- 2015
酶聚合体电流信号放大多通道电化学免疫传感体系及蛋白肿瘤标志物检测
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Abstract:
摘要 本文构建了一种基于酶聚合体信号放大的多通道电化学免疫传感体系，并用于肝癌肿瘤标记物甲胎蛋白（AFP）的定量检测. 该传感体系由固定了抗AFP鼠单克隆抗体的多通道丝网印刷电极组成，可捕获肿瘤标记物抗原AFP，进而与抗AFP兔多抗特异性结合形成夹心免疫复合物，然后利用辣根过氧化物酶聚合体偶联的羊抗兔二抗（IgG-polyHRP）与三明治夹心免疫复合物结合，实现电流信号放大. 该体系结合多通道丝网印刷电极及自主研发的多通道电化学检测仪，可同时满足多通道电流信号的检测. 在最优化条件下，该传感体系检测AFP浓度的动态范围为64 pg·mL-1 ~ 250 ng·mL-1，最低检测下限为56 pg·mL-1，具有检测灵敏度高、特异性强、操作简便以及仪器便携等优点
| [1] | Hu M, Yan J, He Y, et al. Ultrasensitive, multiplexed detection of cancer biomarkers directly in serum by using a quantum dot-based microfluidic protein chip[J]. ACS Nano, 2009, 4(1): 488-494. |
| [2] | Lin J H, Ju H X. Electrochemical and chemiluminescent immunosensors for tumor markers[J]. Biosensors and Bioelectronics, 2005, 20(8): 1461-1470. |
| [3] | Hayes D F, Bast R C, Desch C E, et al. Tumor marker utility grading system: A framework to evaluate clinical utility of tumor markers[J]. Journal of the National Cancer Institute, 1996, 88(20): 1456-1466. |
| [4] | Du D, Zou Z X, Yongsoon S, et al. Sensitive immunosensor for cancer biomarker based on dual signal amplification strategy of graphene sheets and multienzyme functionalized carbon nanospheres[J]. Analytical Chemistry, 2010, 82(7): 2989-2995. |
| [5] | Wang G L, Xu J J, Chen H Y, et al. Label-free photoelectrochemical immunoassay for α-fetoprotein detection based on TiO2/CdS hybrid[J]. Biosensors and Bioelectronics, 2009, 25: 791-796. |
| [6] | Zhou H K, Gan N, Li T H, et al. The sandwich-type electrochemiluminescence immunosensor for α-fetoprotein based on enrichment by Fe3O4-Au magnetic nano probes and signal ampli?cationby CdS-Au composite nanoparticles labeled anti-AFP[J]. Analytica Chimica Acta, 2012, 746: 107-113. |
| [7] | Yang L, Parkin D M, Ferlay J, et al. Estimates of cancer incidence in China for 2000 and projections for 2005[J]. Cancer Epidemiology Biomarkers & Prevention, 2005, 14(1): 243-250. |
| [8] | Liang G D, Liu S F, Zou G Z. Ultrasensitive immunoassay based on anodic near-infrared electrochemiluminescence from dual-stabilizer-capped CdTe nanocrystals[J]. Analytical Chemistry, 2012, 84(24): 10654-10659. |
| [9] | Zhao L F, Li S G, He J, et al. Enzyme-free electrochemical immunosensor con?gured with Au-Pd nanocrystals and N-doped graphene sheets for sensitive detection of AFP[J]. Biosensors and Bioelectronics, 2013, 49: 222-225. |
| [10] | Yang Z J, Liu H, Zong C. Automated support-resolution strategy for a one-way chemiluminescent multiplex immunoassay[J]. Analytical Chemistry, 2009, 81(13): 5484-5489. |
| [11] | Wilson M S. Electrochemical immunosensors for the simultaneous detection of two tumor markers[J]. Analytical Chemistry, 2005, 77(5): 1496-1502. |
| [12] | Jeonga Y, Choib K, Kimc J, et al. PDMS micro bead cage reactor for the detection of alpha fetoprotein (AFP)[J]. Sensors and Actuators B, 2008, 128(2): 349-358. |
