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结肠癌HT29多细胞球的培养及其结构与生物学特性的观察

, PP. 1691-1693

Keywords: 结肠癌,多细胞球,细胞黏附,耐药

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Abstract:

目的建立结肠癌体外三维细胞培养模型HT29多细胞球,鉴定并检测其基本生物学特性。方法琼脂糖抑制细胞贴壁、水平回旋振荡法培养HT29多细胞球;扫描电镜、HE染色、免疫组化检测多细胞球的组织病理学性质;透射电镜检测细胞超微结构;Cellcountingkit-8检测药物敏感性。结果成功培养由细胞相互黏附形成的多层细胞构成的结肠癌多细胞球模型;多细胞球内细胞接触广泛而紧密,可见大量细胞连接;多细胞球局部可见腺腔样结构,细胞角蛋白表达强弱与腺腔样结构有关;多细胞球药物敏感性显性降低,在500μg/L奥沙利铂处理48h条件下,单层细胞生存分数为(9.3±2.0)%,而多细胞球则为(42.9±4.0)%,二者相比差异有统计学意义(P<0.05)。结论成功建立了结肠癌多细胞球模型,此模型是研究体内无血管的微小结肠癌及结肠癌无血管区的较理想模型。

References

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