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肝癌细胞对树突状细胞白介素-12表达的影响

, PP. 1305-1307

Keywords: 树突细胞,,肝细胞,白细胞介素,转化生长因子β,血管内皮生长因子

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Abstract:

目的研究肝癌细胞(hepatocellularcarcinomacells,HCCs)微环境对树突状细胞(dendriticcells,DCs)的白介素-12(interleukin-12,IL-12)表达的影响,以进一步理解肿瘤的免疫逃逸机制。方法免疫磁珠法从人外周血分离CD14+单核细胞,加入rhGM-CSF和rhIL-4将单核细胞诱导分化为imDCs,利用rhTNF-α将imDCs诱导为mDCs,imDCs和mDCs与HCCs在Transwell中共培养48h,ELISA法分别检测不同培养条件下上清液中IL-10、IL-12、转化生长因子β1(trasnformedgrowthfactor-β1,TGF-β1)和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)的表达量。结果与HCCs共培养后,imDCs和mDCs的IL-12的表达量分别从(115.076±8.129)pg/ml和(258.346±3.609)pg/ml下调至(17.599±1.757)pg/ml和(6.787±1.123)pg/ml(P<0.05,P<0.01),而且培养上清中的TGF-β1浓度分别从(133.172±3.053)pg/ml和(174.446±3.972)pg/ml上调至(633.025±9.527)pg/ml和(486.806±10.515)pg/ml(P<0.05,P<0.01),VEGF的浓度分别从0pg/ml上调至(830.892±7.564)pg/ml和(858.169±5.419)pg/ml(P<0.01)。结论HCCs可抑制DCs的IL-12的表达,这可能是HCCs损伤DCs免疫调节功能的方式之一。?

References

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