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放线共生放线杆菌培养上清对成骨细胞分化及间隙连接通讯的影响

, PP. 1059-1063

Keywords: 放线共生放线杆菌,成骨细胞,分化,细胞间隙连接通讯

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Abstract:

目的探讨放线共生放线杆菌(actinobacillusactinomycetemcomitans,A.a)培养上清对成骨细胞分化以及细胞间隙连接通讯的影响。方法在A.a培养上清中体外培养成骨细胞,中性红检测成骨细胞活性,RT-PCR检测成骨细胞分化标记,免疫组化观察Ⅰ型胶原蛋白表达,利用划痕标记荧光染料示踪(SLDT)法与Westernblot检测Connexin43的表达,研究成骨细胞间隙连接通讯的变化。结果20%以下浓度的A.a培养上清对成骨细胞无明显致死性。RT-PCR结果显示A.a培养上清中成骨细胞分化标记的表达降低。免疫组化结果显示A.a培养上清中成骨细胞Ⅰ型胶原蛋白表达降低。SLDT法结果显示A.a培养上清中成骨细胞荧光染料扩散低于对照组。Westernblot检测结果显示A.a培养上清中成骨细胞Connexin43表达降低。结论A.a培养上清对成骨细胞分化及细胞间隙连接通讯有抑制作用。

References

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