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体外树突状细胞通过NT-3上调P-ERK1/2的表达促进神经干/祖细胞分化

, PP. 2368-2372

Keywords: 干细胞,树突状细胞,共培养,分化,NT-

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Abstract:

目的观察树突状细胞(dendriticcells,DCs)对神经干/祖细胞(neuralstem/progenitorcells,NSPCs)分化的影响,探讨神经营养素-3(Neurotrophin-3,NT-3)在DCs调控NSPCs分化中可能的作用机制。方法实验共设5组,分别为NSPCs组、NSPCs/DCs组、NSPCs+NT-3组、NSPCs/DCs+抗NT-3组和DCs组。共培养24、48、72h后,ELISA法检测各组上清液中NT-3含量;7d后荧光免疫细胞化学法检测各组NSPCs分化情况。Westernblot检测各组NSPCs磷酸化的细胞外调节蛋白激酶(extracellularregulatedproteinkinases,ERK1/2)表达。结果NSPCs/DCs组上清液中NT-3含量较NSPCs组、NSPCs/DCs+抗NT-3组和DCs显著升高(P<0.05)。NSPCs/DCs组和NSPCs+NT-3组β-tubulin-Ⅲ阳性细胞数较NSPCs组和NSPCs/DCs+抗NT-3组显著增高(P<0.05),而NSPCs组和NSPCs/DCs+抗NT-3组GFAP阳性细胞较NSPCs/DCs组和NSPCs+NT-3组显著增高(P<0.05)。NSPCs/DCs组和NSPCs+NT-3组NSPCs中的p-ERK1/2的表达较其余两组增高(P<0.05)。结论NSPCs与DCs共培养能显著促进NSPCs分化为神经元,可能与共培养后NT-3表达量增高,通过其特异性受体TrkC激活下游信号通路MEK-ERK有关。

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