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SIRT2基因沉默后对肝癌细胞增殖的影响

, PP. 2098-2102

Keywords: SIRT,肝癌,增殖

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Abstract:

目的探讨沉默信息调节因子2相关酶2(silentinformationregulator2relatedenzyme2,SIRT2)基因沉默对肝癌细胞增殖的影响及分子机制。方法利用慢病毒介导的shRNA干扰技术,靶向沉默SIRT2基因的表达;Westernblot检测SIRT2基因沉默效果及SIRT2沉默后β-catenin蛋白的表达变化;流式细胞计数检测SIRT2基因沉默后肝癌细胞周期的变化,CCK-8法检测SIRT2基因沉默对肝癌细胞增殖的影响,台盼蓝计数检测SIRT2基因沉默及过表达β-catenin后,SIRT2基因沉默对肝癌细胞增殖的影响;BrdU实验检测SIRT2基因沉默对肝癌细胞DNA合成的影响;平板集落实验检测SIRT2沉默对肝癌细胞克隆形成能力的影响。结果SIRT2shRNA靶向沉默显著抑制肝癌细胞SIRT2的表达水平,shRNA沉默组肝癌细胞PLC-5的增殖能力较对照组分别下降48.3%(shSIRT2-1)、55.6%(shSIRT2-2)(P<0.01);SIRT2基因沉默后,肝癌细胞出现G1期阻滞,β-catenin蛋白表达水平下降;过表达β-catenin后SIRT2基因沉默组较对照组肝癌细胞增殖分别升高了89%(shSIRT2-1)、75%(shSIRT2-2)(P<0.01)。结论SIRT2沉默可能通过调节β-catenin信号通路来影响肝癌细胞增殖。

References

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