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骨诱导膜活化灭活骨修复骨缺损的实验研究

, PP. 2061-2064

Keywords: 诱导膜,骨重建,灭活骨,间充质干细胞

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Abstract:

目的构建骨缺损诱导膜模型,探讨骨诱导膜对灭活移植骨成骨诱导活性的作用。方法32只新西兰大白兔一期在桡骨中段制造骨缺损模型,植入聚甲基丙烯酸甲酯骨水泥诱导生物膜形成。第6、8周分别处死4只取出诱导膜检测骨形态发生蛋白(bonemorphogeneticprotein-2,BMP-2)和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)含量并切片作HE染色,取同只兔皮下组织作为对照。其余24只兔在一期植入骨水泥后6周取出骨水泥进行二期手术,按随机数字表法分为实验组、模型组和对照组,每组8只。实验组保留诱导膜并在膜内植入灭活骨,模型组保留诱导膜不植骨,对照组去除诱导膜植入灭活骨。二期处理后第8、12周通过放射学和组织学评估新生骨形成情况。结果ELISA检测表明在第6、8周诱导膜中BMP-2含量[(437.40±20.75)、(346.67±30.05)pg/mL]、VEGF含量[(362.33±38.85)、(233.84±18.20)pg/mL]均高于皮下组织(P<0.05),同时在诱导膜切片HE染色中观察到软骨内骨化;放射学检测可见二期处理后第8、12周实验组成骨优于模型组和对照组(P<0.01),模型组有少许骨痂形成,对照组无成骨;第8、12周组织学观察实验组可见骨细胞和软骨细胞形成,成骨优于模型组和对照组(P<0.01),对照组仅有纤维连接,未见骨细胞和软骨细胞。结论骨诱导膜含有间充质干细胞和高浓度的细胞因子,从而促进灭活移植骨重建骨缺损。

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