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RhoC-siRNA表达载体对肝癌细胞生长速度及侵袭力的影响

, PP. 1779-1782

Keywords: RhoC,siRNA,肝癌细胞,侵袭

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Abstract:

目的构建RhoC-siRNA表达载体,研究其对肝癌细胞生物学行为的影响。方法构建RhoC-siRNA表达载体,以脂质体转染法将之稳定转染入SK-Hep1肝癌细胞株,Westernblot法鉴定转染细胞中RhoC蛋白表达的抑制情况,观察转染前后肝癌细胞形态、生长曲线、黏附、运动及体外侵袭能力的改变。结果酶切及测序证实RhoC-siRNA真核表达载体构建成功,将之转染入肝癌细胞后,细胞中RhoC蛋白表达水平降低达60%,细胞形态及生长速度无明显改变,但细胞黏附、运动能力及体外侵袭能力均降低。结论RhoC-siRNA表达载体对肝癌细胞的形态及生长曲线无显著影响,但可抑制其体外侵袭转移能力,可为肝癌的生物学治疗提供新的方法。

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