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HIF-1α条件性基因敲除嵌合体小鼠的获得

, PP. 1361-1363

Keywords: 低氧诱导因子-α,条件性基因敲除,同源重组,胚胎干细胞,Cre/loxP系统

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Abstract:

目的应用Cre/loxP系统建立HIF-1α条件性基因敲除小鼠模型。方法电击法将构建好的HIF-1α条件性基因敲除载体转染入小鼠ES细胞,用G418与Ganciclovir对转染的ES细胞进行正负筛选,经Southern杂交鉴定得到阳性ES细胞克隆后,将已发生正确重组的ES细胞显微注射入小鼠囊胚腔,对子代鼠进行基因型鉴定。结果经G418与Ganciclovir筛选培养得到490个阳性克隆,用5′端探针进行Southern杂交鉴定获得1株发生同源重组的ES细胞克隆,经显微注射,得到5只由基因敲除ES细胞和供体囊胚细胞共同发育而来的嵌合体小鼠。结论成功得到基于Cre/loxP系统的HIF-1α条件性基因敲除嵌合体小鼠模型。

References

[1]  赵玲,杜晓兰,苏楠,等. HIF-1α条件性基因敲除嵌合体小鼠的获得[J]. 第三军医大学学报, 2007, 29(14):1361-1363.
[2]  李福兵,赵玲,鲁秀敏,等.成熟成骨细胞中敲除基因fgfr1小鼠的制备[J].第三军医大学学报,2008,30(04):280.  LI Fu-bing,ZHAO Ling,LU Xiu-min,et al.Generation of differentiated osteoblast specific fgfr1 knockout mice[J].J Third Mil Med Univ,2008,30(14):280. [2]王金良,孔佩艳,徐葳,等.人参皂苷Rg3抑制急性白血病骨髓基质细胞HIF-1α及VEGF的表达及其机制探讨[J].第三军医大学学报,2010,32(07):621.  Wang Jinliang,Kong Peiyan,Xu Wei,et al.Ginsenosisde Rg3 inhibits HIF-1α and VEGF expressions in acute leukemia bone marrow stromal cells[J].J Third Mil Med Univ,2010,32(14):621. [3]王雯,陈森洲,王险峰,等.HIF-1α、VEGF在CIA大鼠模型中的表达与意义[J].第三军医大学学报,2010,32(06):563.  Wang Wen,Chen Senzou,Wang Xianfen,et al.Expression and significance of HIF-1α and VEGF in rats with collagen Ⅱ-induced arthritis[J].J Third Mil Med Univ,2010,32(14):563. [4]高兵,简华刚,曾勇,等.负压创缘治疗对慢性创缘HIF-1α和VEGF表达的影响[J].第三军医大学学报,2009,31(10):941.  GAO Bing,JIAN Hua-gang,ZENG Yong,et al.Negative pressure wound treatment upregulates hypoxia inducible factor-1α and VEGF expressions during wound healing[J].J Third Mil Med Univ,2009,31(14):941. [5]周炜,姜政,张滨,等.短发夹RNA干扰胃癌低氧诱导因子-1α表达及其临床意义[J].第三军医大学学报,2009,31(02):152.  ZHOU Wei,JIANG Zheng,ZHANG Bin,et al.RNA interference targeting hypoxia inducible factor-1α in BGC-823 cells inhibits cell proliferation and induces cell apoptosis[J].J Third Mil Med Univ,2009,31(14):152. [6]谢宜军,王月刚,郭寿贵,等.携EGFP的人低氧诱导因子-1α腺病毒载体的构建及鉴定[J].第三军医大学学报,2007,29(02):117.  XIE Yi-jun,WANG Yue-gang,GUO Shou-gui,et al.Construction and identification of adenovirus vector of human hypoxia-inducible factor-1α fused with enhanced green fluorescent protein[J].J Third Mil Med Univ,2007,29(14):117. [7]贾朝莉,龙方懿,刘智敏,等.氯化钴对甲状腺乳头状癌NPA细胞上皮间质转化的影响及其机制[J].第三军医大学学报,2012,34(02):150.  Jia Chaoli,Long Fangyi,Liu Zhimin,et al.CoCl2 improves epithelial-mesenchymal transition of human papillary thyroid carcinoma cells[J].J Third Mil Med Univ,2012,34(14):150. [8]周年,黄伟,廖军义,等.低氧诱导因子-1α对骨形态发生蛋白2诱导的干细胞成软骨、成骨分化的影响[J].第三军医大学学报,2014,36(12):1243.  Zhou Nian,Huang Wei,Liao Junyi,et al.HIF-1α potentiates BMP2-induced chondrogenic differentiation but inhibits osteogenic differentiation in stem cells[J].J Third Mil Med Univ,2014,36(14):1243.

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