全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

缺氧对ECV-304细胞CASK表达及细胞内分布的影响

, PP. 1142-1144

Keywords: 缺氧,钙/钙调蛋白依赖性丝氨酸蛋白激酶,ECV-细胞

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的了解缺氧后ECV-304细胞钙/钙调蛋白依赖性丝氨酸蛋白激酶(calcium/calmodulin-dependentserineproteinkinase,CASK)蛋白表达及细胞内分布的变化。方法将培养的ECV-304细胞置于缺氧(5%O2)条件培养1、3、6、12h,用Westernblot方法检测CASK表达的变化,用免疫组化技术观察CASK在细胞内分布情况。结果ECV-304细胞的CASK表达随缺氧时间延长而逐渐增高,3h开始增加,6h达高峰,12h下降但仍高于正常。免疫组织化学染色的结果显示,随着缺氧时间的延长,细胞染色加深、并出现CASK向核膜聚集的现象。结论缺氧可以影响CASK的蛋白表达和细胞内分布,提示CASK可能是一个新的值得关注的缺氧相关蛋白。

References

[1]  陈建,梁光萍,苏踊跃,等. 缺氧对ECV-304细胞CASK表达及细胞内分布的影响[J]. 第三军医大学学报, 2007, 29(12):1142-1144.
[2]  周帅,蒋理,程崇杰,等.载脂蛋白E对星形胶质细胞缺氧性损伤的保护作用[J].第三军医大学学报,2012,34(20):2067.  Zhou Shuai,Jiang Li,Cheng Chongjie,et al.Apolipoprotein E protects hypoxic injuried astrocytes in vitro[J].J Third Mil Med Univ,2012,34(12):2067. [2]生宝亮,徐刚,陈德伟,等.大鼠实验性高原肺水肿中T-AOC、MDA、SOD、CAT和IL-6的表达[J].第三军医大学学报,2012,34(23):2364.  Sheng Baoliang,Xu Gang,Chen Dewei,et al.T-AOC,MDA,SOD,CAT and IL-6 levels in rat pulmonary edema induced by hypobaric hypoxia[J].J Third Mil Med Univ,2012,34(12):2364. [3]邹哲华,陶陶,徐坚,等.大鼠大脑皮层神经元缺氧后细胞凋亡情况的动态观察[J].第三军医大学学报,2012,34(24):2489.  Zou Zhehua,Tao Tao,Xu Jian,et al.Dynamic changes of apoptosis in rat cerebral cortex neurons after hypoxia[J].J Third Mil Med Univ,2012,34(12):2489. [4]魏勇,顾健腾,鲁开智,等.瑞芬太尼对肝细胞缺氧复氧损伤保护作用的机制研究[J].第三军医大学学报,2007,29(20):1976.  WEI Yong,GU Jian-teng,LU Kai-zhi,et al.Remifentanyl protects hepatocytes against anoxia-reoxygenation injury[J].J Third Mil Med Univ,2007,29(12):1976. [5]杨兆瑞,吴晴,陈嘉薇,等.HIF-1α、JNK1、P-gp、MRP1和LRP蛋白在胃癌中的表达与临床病理及预后的关系[J].第三军医大学学报,2007,29(18):1755.  YANG Zhao-rui,WU Qing,CHEN Jia-wei,et al.Expressions, clinicopathological features and prognostic significance of HIF-1α, JNK1, P-gp, MRP1 and LRP proteins in human gastric carcinoma[J].J Third Mil Med Univ,2007,29(12):1755. [6]罗红,高文祥,高钰琪,等.茶多酚预防缺氧大鼠红细胞增多症的实验研究[J].第三军医大学学报,2007,29(14):1381.  LUO Hong,GAO Wen-xiang,GAO Yu-qi,et al.Protective effects of tea polyphenols on polycythemia induced by chronic hypoxia in rats[J].J Third Mil Med Univ,2007,29(12):1381. [7]刘茜,吴亚梅,徐玲,等.急性缺氧诱导C57BL/6J小鼠瘦素表达增加[J].第三军医大学学报,2007,29(13):1311.  LIU Xi,WU Ya-mei,XU Ling,et al.Influence of hypoxia on leptin expression in C57BL/6J mice[J].J Third Mil Med Univ,2007,29(12):1311. [8]张钢,高钰琪,刘福玉.缺氧对大鼠白细胞粘弹性的影响[J].第三军医大学学报,2008,30(01):46.  ZHANG Gang,GAO Yu-qi,LIU Fu-yu.Effects of hypoxia on viscoelastic properties of polymorphonuclear leukocyte of rats[J].J Third Mil Med Univ,2008,30(12):46. [9]陈建,黄缄,高钰琪,等.预缺氧对大鼠低压缺氧性脑损伤保护作用的实验研究[J].第三军医大学学报,2007,29(22):2113.  CHEN Jian,HUANG Jian,GAO Yu-qi,et al.Protective effects of hypoxia preconditioning on rat brain against hypobaric hypoxia[J].J Third Mil Med Univ,2007,29(12):2113. [10]吴喜贵,赵延东,阮怀珍.缺氧对大鼠皮层、海马NMDA受体NR1亚单位磷酸化的影响[J].第三军医大学学报,2007,29(18):1742.  WU Xi-gui,ZHAO Yan-dong,RUAN Huai-zhen.Effect of hypoxia on NR1 subunit of NMDA receptor in rat cortex and hippocampus[J].J Third Mil Med Univ,2007,29(12):1742. [11]苏踊跃,孙林辉,邬江,等.CASK在血管内皮细胞缺氧应激增殖反应中的作用[J].第三军医大学学报,2010,32(14):1546.  Su Yongyue,Sun Linhui,Wu Jiang,et al.Role of calcium/calmodulin-dependent serine protein kinase in hypoixa related endothelial cell proliferation[J].J Third Mil Med Univ,2010,32(12):1546.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133