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成年豚鼠膀胱ICCs细胞起搏电流的鉴定

, PP. 567-569

Keywords: Cajal间质细胞,起搏电流,膀胱

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Abstract:

目的确定成年豚鼠膀胱卡哈尔间质细胞(interstitialcellsofCajal,ICCs)细胞是否具有起搏电流。方法采用胶原酶消化法,得到原代培养的成年豚鼠膀胱ICCs细胞。采用膜片钳技术,在全细胞记录模式下,记录其超级化激活的内向电流(hyperrepolarization-activatedinwardcurrent,Ih)。结果原代培养的ICCs细胞和逼尿肌细胞的静息膜电位、膜电容存在明显差异;ICCs细胞可记录到起搏细胞特征电流Ih,而在逼尿肌细胞则记录不到。结论记录到成年豚鼠膀胱ICCs细胞具有起搏细胞的特征电流Ih,提示ICCs细胞在膀胱具有起搏作用。

References

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