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大鼠EPO真核表达载体的构建及其在骨髓间充质干细胞中的表达

, PP. 2186-2188

Keywords: 促红细胞生成素,真核表达载体,骨髓间充质干细胞,缺氧缺血性脑病

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Abstract:

目的构建表达大鼠促红细胞生成素(erythropoietin,EPO)的真核表达质粒pEGFP-N1/EPO,并检测其在体外的表达。方法从大鼠肾脏获得EPO基因片段并用RT-PCR方法扩增,将其连接于真核表达质粒pEGFP-N1,测序鉴定后,用脂质体包裹转染大鼠骨髓间充质干细胞(mesenchymalstemcells,MSCs),采用免疫组化和RT-PCR检测EPO的表达。结果经酶切和测序鉴定证实本实验构建的重组表达质粒正确,该质粒在体外转染大鼠MSCs细胞后可表达EPO蛋白。结论成功构建的pEGFP-N1/EPO重组质粒能在体外表达EPO蛋白。

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