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人Snail基因RNAi慢病毒载体对鼻咽癌5-8F细胞增殖和侵袭的影响

, PP. 1151-1154

Keywords: Snail,RNA干扰,鼻咽癌,增殖,侵袭

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Abstract:

目的构建人Snail基因慢病毒干扰载体及观察干扰前后对鼻咽癌5-8F细胞增殖和侵袭的影响。方法设计Snail基因特异性RNAi靶序列,克隆至经双酶切后的plVTHM线性化载体,测序鉴定正确后,用病毒上清感染鼻咽癌细胞5-8F,流式细胞仪分选获得稳定干扰Snail基因的细胞亚系。荧光定量PCR检测mRNA表达情况;MTT法和体外侵袭实验分别测定对细胞增殖和侵袭的影响。结果plVTHM-siSnail慢病毒干扰载体构建正确。荧光定量PCR结果表明分选后的细胞SnailmRNA水平表达显著降低。干扰后的细胞增殖减慢,穿透基膜的细胞数量减少,差异具有统计学意义(P<0.05)。结论plVTHM-siSnail表达质粒可显著下调Snail基因在5-8F中的表达,在一定程度上抑制鼻咽癌细胞的增殖和侵袭。

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