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clpP基因缺陷对肺炎链球菌毒力表达降低的研究

, PP. 307-310

Keywords: 肺炎链球菌,毒力因子,热休克蛋白,clpP基因

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Abstract:

目的探索clpP基因缺陷对肺炎链球菌(Streptococcuspneumoniae,S.pn)毒力的影响。方法用长臂同源多聚酶链式反应(LFH-PCR)方法失活clpP基因,用PCR、Westernblot鉴定缺陷菌株,通过RT-PCR分析自溶素(majorautolysinA,lytA)、表面黏附A(pneumococcalsurfaceadhesionA,psaA)、溶血素(pneumolysin,ply)和胆碱结合蛋白A(cholinebindingproteinA,cbpA)的表达,并通过动物实验观察clpP基因缺陷株毒力改变情况。结果RT-PCR显示clpP缺陷株的4个毒力因子mRNA表达水平均低于野生菌,两者比较有统计学差异(P<0.05);小鼠毒力实验表明野生菌株半数致死时间为22h,而缺陷株半数致死时间为8d,两者比较有统计学差异(P<0.01);缺陷菌在小鼠体内被清除速度也显著快于野生菌株(P<0.05)。结论clpP基因缺陷使S.pn多种毒力因子表达减弱,毒力降低。

References

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