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载脂蛋白E基因多态性影响星形胶质细胞损伤后早期NF-κB表达的实验研究

, PP. 103-106

Keywords: 载脂蛋白E,基因多态性,星形胶质细胞,核因子kappaB

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Abstract:

目的探讨载脂蛋白E(apolipoproteinE,APOE)基因多态性与星形胶质细胞损伤后NF-κB表达的相关性。方法通过RT-PCR及定点突变技术扩增人源性APOEε2、ε3、ε43种等位基因的CDS区,并构建pEGFP-N1-APOE重组质粒;APOE基因敲除鼠星形胶质细胞的原代培养、鉴定,脂质体法将重组质粒转染入星形胶质细胞,并筛选稳定表达APOE信息的细胞株;利用划痕致伤构建细胞损伤模型,于伤后12、24、48h,通过RT-PCR测定NF-κBmRNA的转录,通过Westernblot测定其蛋白的表达。结果伤后12h,APOEε2、ε3、ε43组细胞可检测到NF-κB的表达,但各组间比较差异无统计学意义(P>0.05);伤后24h,3组细胞NF-κB的表达较伤前增高,且APOEε4组的表达明显高于APOEε2和APOEε3组(P<0.05);伤后48h,表达进一步增强,APOEε4组的表达依然明显高于APOEε2和APOEε3组(P<0.05)。结论损伤后早期,携带APOEε4等位基因的星形胶质细胞NF-κB的表达水平高于APOEε2和APOEε3型,提示APOEε4携带体可能通过早期创伤性炎症反应导致急性期伤情加重及不良预后。?

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