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缺氧对人小细胞肺癌H446细胞基因组DNA甲基化水平的影响

, PP. 2145-2147

Keywords: 缺氧,,小细胞,DNA甲基化,细胞系,肿瘤

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Abstract:

目的研究缺氧对人小细胞肺癌H446细胞基因组DNA甲基化水平的影响。方法体外培养人小细胞肺癌细胞H446,置于缺氧(3%O2)条件下培养,分别于0、12、24、48、72h后提取细胞基因组DNA,用甲基化敏感性随机引物PCR(MS-AP-PCR)方法检测基因组DNA甲基化状态。结果在缺氧状态下,H446细胞经酶解后的扩增谱带明显增多,且荧光强度较强,以24h后明显,表明H446细胞基因组DNA甲基化水平在缺氧24h后即发生明显升高,主要表现为多位点的异常甲基化。结论缺氧可能是造成肿瘤细胞内甲基化失衡的重要原因之一。

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