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内质网应激在软脂酸钠诱导的脂肪变性L02肝细胞凋亡中的作用

, PP. 1935-1938

Keywords: 软脂酸钠,内质网应激,非酒精性脂肪性肝病,凋亡

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Abstract:

目的观察内质网应激(endoplasmicreticulumstress,ERS)蛋白在软脂酸钠诱导的脂肪变性L02肝细胞凋亡过程中的改变,探讨ERS与脂变肝细胞凋亡的关系。方法用软脂酸钠(饱和脂肪酸)诱导建立L02肝细胞脂肪变性模型。将细胞分为正常对照组和实验组(软脂酸钠72μmol/L)。MTT法筛选软脂酸钠最佳作用浓度;油红O染色和甘油三酯(TG)试剂盒检测细胞内脂变程度;流式细胞术AnnexinV-PE/7-AAD双染检测细胞凋亡率;Westernblot检测GRP78、CHOP、磷酸化JNK(p-JNK)蛋白表达。结果软脂酸钠在体外能够诱导肝细胞发生脂肪变性以及脂变肝细胞凋亡,与对照组(1.38±0.42)比较,实验组48h细胞凋亡率(5.95±0.37)显著增加(P<0.05)。Westernblot检测示软脂酸钠刺激肝细胞12h,GRP78表达(0.50±0.03)较0h(0.37±0.02)明显增加,48h(0.83±0.01)达高峰(P<0.05)。CHOP和p-JNK表达与GRP78几乎同步增加。结论软脂酸钠对L02肝细胞具有较强的脂毒性,可以诱导肝细胞发生ERS反应。ERS上调CHOP和P-JNK表达可能在软脂酸钠诱导脂肪变性肝细胞凋亡中具有重要作用。

References

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