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Bcl-2和Bax在小鼠海马发育中的表达变化

, PP. 1831-1833

Keywords: 海马,Bcl-,Bax,小鼠

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Abstract:

目的探讨Bcl-2和Bax在小鼠海马发育过程中的表达变化。方法利用免疫组织化学及图像分析技术对小鼠海马发育中Bcl-2和Bax蛋白进行分析。结果Bcl-2免疫组化结果:胚龄(embryonicday,E)16dCA1区有少量阳性细胞。E18d,海马各区均可见阳性细胞,以锥体层居多。生后(postnatalday,P)1~21d,海马各区阳性细胞先密集后稀疏,以P7d最密集,CA3区最密集。P28d后趋于稳定。锥体细胞层Bcl-2阳性表达细胞的平均光密度(AOD)值先增加后减小,P7d为最大值(P<0.01),P28d后趋于稳定(P>0.05)。Bax表达结果与Bcl-2结果相似。Bcl-2/Bax比值E18d~P1d逐渐降低(P<0.01),之后趋于稳定(P>0.05)。结论Bcl-2和Bax在胚胎期和生后早期小鼠海马有高表达,其凋亡高峰是生后1d。成年及老年后保持低水平。

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