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Nek2基因沉默对卵巢癌SKOV3细胞侵袭能力的影响

, PP. 1514-1517

Keywords: NIMA相关激酶,RNA干扰,卵巢癌,侵袭

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Abstract:

目的研究RNA干扰Nek2表达对卵巢癌SKOV3细胞侵袭能力的影响以及相关的分子机制。方法设计合成3条针对Nek2基因的siRNA,转染至SKOV3细胞中,采用Real-timeRT-PCR和Westernblot技术筛选出抑制效率最高的Nek2-siRNA序列,用Transwell方法检测转染该序列的SKOV3细胞侵袭能力的改变,并用Westernblot方法检测Nek2-siRNA转染后SKOV3细胞中MMP-2、MMP-9和TIMP-1蛋白表达的变化。结果①RNA干扰序列2对SKOV3细胞中Nek2的抑制效果最明显;②与未转染组和阴性对照组相比,转染了Nek2-siRNA的SKOV3细胞侵袭能力明显下降(P<0.01);③Westernblot检测表明,与未转染组和阴性对照组相比,Nek2-siRNA转染48h后SKOV3细胞中MMP-2、MMP-9的表达明显下调,而TIMP-1的表达明显上调(P<0.01)。结论Nek2-siRNA能有效得沉默SKOV3细胞中Nek2的表达,通过下调MMP-2、MMP-9的表达,上调TIMP-1的表达,从而抑制SKOV3细胞的侵袭转移。

References

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