全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

RNA干扰PDK-1对5-FU诱导结肠癌细胞凋亡的促进作用

, PP. 9-12

Keywords: 结肠肿瘤,丙酮酸脱氢酶激酶-,细胞增殖,化学治疗,凋亡

Full-Text   Cite this paper   Add to My Lib

Abstract:

目的探讨以丙酮酸脱氢酶激酶-1(pyruvatedehydrogenasekinaseisozyme1,PDK-1)为靶标的短发夹RNA(shRNA)与化疗药5-氟尿嘧啶(5-FU)联合应用对人结肠癌LS174T细胞的促凋亡作用。方法针对PDK-1序列设计3段干扰序列及1个阴性对照,分别构建pGenesil.1-PDK-1重组质粒表达载体及pGenesil.1-NC阴性对照载体,利用脂质体分别将质粒转染入LS174T,并用G418对细胞进行药物筛选,并通过Real-timePCR和Westernblot检测有干扰序列的有效性。MTT实验检测5-FU对干扰组和对照组细胞生长、增殖的影响,流式细胞仪检测细胞凋亡率。结果Real-timePCR和Westernblot证实3个干扰组均抑制PDK-1表达,其中最大敲减效率超过70%。MTT实验结果显示,经5-FU同样处理48h后,PDK-1干扰的LS174T细胞生长抑制率较对照组显著增高(P<0.05);流式细胞仪结果显示,干扰组细胞凋亡率较对照组增高(P<0.05)。结论PDK-1shRNA能特异性下调LS174T细胞中PDK-1的表达,PDK-1干扰联合5-FU可促进结肠癌细胞化疗诱导凋亡。

References

[1]  谢?丰, 梁后杰, 童晶涛, 等. RNA干扰PDK-1对5-FU诱导结肠癌细胞凋亡的促进作用[J].第三军医大学学报,2012,34(1):9-12.
[2]  李晓桓,朱蓉,田音,等.干扰结肠癌上皮细胞Ascl2的表达致其向杯状细胞表型分化[J].第三军医大学学报,2012,34(19):1936.  Li Xiaohuan,Zhu Rong,Tian Yin,et al.Interference of Asc12 expression in colon cancer epithelial cells leads to their differentiation into phenotype of goblet cells[J].J Third Mil Med Univ,2012,34(01):1936. [2]彭亦良,梁后杰,高晋华,等.CXCR4和VEGF-C在结肠癌组织中的表达及与淋巴结转移之间的关系[J].第三军医大学学报,2006,28(04):357. [3]黎川,陈伟,黎海涛,等.多排螺旋CT仿真结肠镜对结直肠占位性病变诊断价值的研究[J].第三军医大学学报,2006,28(19):1994. [4]廖新华,陈祖林,葛海燕,等.低浓度的丝裂霉素对结肠癌光动力增效作用研究[J].第三军医大学学报,2005,27(20):2054. [5]李前伟,厉红民,黎川,等.18F-FDG PET/CT显像诊断结肠癌伴肝多发转移1例[J].第三军医大学学报,2005,27(14):1496. [6]尚立娜,杨爱军,王晨昱,等.内皮细胞对结肠癌细胞系SW480中CD133+细胞表达VEGF的影响[J].第三军医大学学报,2010,32(16):1740.  Shang Lina,Yang Aijun,Wang Chenyu,et al.Effect of endothelial cells on VEGF expression in CD133+ cells from SW480 cells[J].J Third Mil Med Univ,2010,32(01):1740. [7]刘美,李少林,陈宓,等.人结肠癌细胞株CW-2干细胞富集方法的比较[J].第三军医大学学报,2010,32(09):912.  Liu Mei,Li Shaolin,Chen Mi,et al.Comparison on enriching methods for colon cancer stem cells from human colon cancer cell line CW-2[J].J Third Mil Med Univ,2010,32(01):912. [8]童晶涛,梁后杰,陈建芳,等.丙酮酸脱氢酶激酶-1在人结肠癌中的表达及意义[J].第三军医大学学报,2010,32(03):257.  Tong Jingtao,Liang Houjie,Chen Jianfang,et al.Expression of pyruvate dehydrogenase kinase-Ⅰ in human colon cancer and its significance[J].J Third Mil Med Univ,2010,32(01):257. [9]萨日娜,欧娟娟,耿培亮,等.上皮-间质转化在人结肠癌细胞系SW480对西妥昔单抗及氟尿嘧啶耐药中的作用[J].第三军医大学学报,2011,33(08):776.  Sa Rina,Ou Juanjuan,Geng Peiliang,et al.Role of epithelial-mesenchymal transition in drug resistance of human colon cancer cell line SW480 to cetuximab and 5-fluorouracil[J].J Third Mil Med Univ,2011,33(01):776. [10]王攀,张超,刘涛,等.CLIC-1 mRNA及蛋白在结肠癌的表达及临床意义[J].第三军医大学学报,2011,33(22):2407.  Wang Pan,Zhang Chao,Liu Tao,et al.mRNA and protein expression of chloride intracellular channel 1 in colon cancer and clinical significance[J].J Third Mil Med Univ,2011,33(01):2407.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133