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miR-145在间充质干细胞来源软骨细胞肥大化中的作用

, PP. 1058-1061

Keywords: 间充质干细胞,软骨细胞,肥大化,miR-,Sox

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Abstract:

目的探讨miR-145对间充质来源软骨细胞肥大化的调控作用。方法用肥大化完全诱导培养基对间充质干细胞进行软骨细胞肥大化诱导,检测肥大化相关基因表达,确定间充质来源软骨细胞肥大化诱导成功。实验分为转染antagomiR-145(miRNA拮抗剂)组和对照组,应用qRT-PCR检测miR-145对软骨细胞肥大化标志基因Ⅹ型胶原(ColⅩ)、成纤维细胞生长因子受体-1(FGFR-1)和基质金属蛋白酶-13(MMP-13)转录水平的影响。结果转染antagomiR-145可以延缓间充质来源软骨细胞肥大化进程。转染antagomiR-145后肥大化软骨细胞的标志物ColⅩ、FGFR-1、MMP-13的表达量与对照组相比显著降低(P<0.05),而ColⅡ的表达与对照组相比显著增多(P<0.05)。结论抑制miR-145的表达能有效地延缓间充质干细胞向肥大化软骨细胞分化进程。

References

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