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IBP表达抑制后活化T淋巴细胞基因表达谱变化分析

, PP. 109-113

Keywords: 干扰素调节因子-结合蛋白,T淋巴细胞,基因芯片

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Abstract:

目的利用全基因组寡核苷酸芯片检测干扰素调节因子-4结合蛋白(IRF-4bindingprotein,IBP)表达抑制后活化T淋巴细胞基因表达谱的差异。方法采用本室构建的IBP表达抑制的JurkatT细胞及相应对照细胞为实验对象,Anti-CD3、CD28mAb处理细胞后提取刺激24、48h的细胞总RNA,利用北京博奥生物有限公司22KHumanGenomeArray芯片检测基因表达的差异,以MAS.doc.V1软件,结合KEGG、NCBI等生物信息学数据库检索分析差异表达基因功能和网络关系。结果IBP基因表达抑制的JurkatT细胞在TCR信号刺激24、48h后,细胞能量代谢、周期生长、转录调控及凋亡等多种类型的基因发生差异表达改变,2个时相组共有56个差异表达趋势一致基因,其中17个基因共同上调,39个基因共同下调。结论在活化的JurkatT细胞中IBP表达抑制所致多个基因差异表达。

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