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反义RNA抑制c-myc表达增强骨肉瘤细胞对顺铂的敏感性

, PP. 2463-2466

Keywords: 反义c-myc,骨肉瘤,顺铂,细胞凋亡

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Abstract:

目的通过对骨肉瘤细胞MG-63和U2OSc-myc基因干扰,初步探讨顺铂对c-myc低表达骨肉瘤细胞凋亡的影响。方法以腺病毒为载体,构建表达反义c-myc的重组腺病毒(Ad-Asc-myc),并在体外感染骨肉瘤细胞MG-63和U2OS,与不同浓度及不同时间点的顺铂作用后,采用Westernblot方法检测干扰水平;瑞氏染色和MTT检测顺铂对骨肉瘤细胞MG-63和U2OS凋亡作用。结果构建包装的腺病毒Ad-Asc-myc体外感染MG-63和U2OS48h后,可明显降低c-myc蛋白表达;随着顺铂浓度和处理时间增加可增强对细胞增殖的抑制。结论抑制c-myc的表达可以增强骨肉瘤对化疗的敏感性。

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