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雷帕霉素诱导细胞自噬促进乙型肝炎病毒的复制

, PP. 1233-1236

Keywords: 雷帕霉素,自噬,乙型肝炎病毒,复制

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Abstract:

目的研究雷帕霉素诱导细胞产生自噬对乙型肝炎病毒(hepatitisBvirus,HBV)复制的影响。方法通过雷帕霉素诱导人肝癌细胞HepG22.15产生自噬,荧光显微镜和透射电镜下观察自噬小体形成,Westernblot检测诱导后HepG22.15细胞自噬标志蛋白LC3-II/LC3-I的表达水平;荧光定量PCR检测HBVmRNA水平;加入自噬抑制剂3-甲基腺嘌呤(3-MA),Westernblot检测自噬抑制后LC3-II/LC3-I的表达水平,酶联免疫吸附试验(ELISA)检测乙肝病毒表面抗原(HBsAg)的表达,Southernblot检测HBVDNA水平,验证HBV复制情况。结果雷帕霉素诱导后,HepG22.15细胞在荧光显微镜和透射电镜下观察到自噬小体的形成,LC3-II/LC3-I比值显著上升(P<0.05);细胞诱导自噬后HBsAg和HBVmRNA显著增加(P<0.05),而加入自噬抑制剂3-MA后,HBsAg和HBVDNA均显著减少(P<0.05)。结论雷帕霉素可能通过诱导细胞自噬促进HBV的复制。

References

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