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miR-200c下调KRAS基因表达抑制膀胱癌细胞体外增殖能力

, PP. 2142-2146

Keywords: 膀胱癌,miR-c,KRAS,增殖

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Abstract:

目的探讨miR-200c对膀胱癌细胞体外增殖能力的作用及机制。方法慢病毒感染法建立稳定过表达和稳定低表达miR-200c膀胱癌细胞,分为对照组、稳定过表达miR-200c组(miR-200c组)、稳定低表达miR-200c组(miR-200c-inhibit组),实时定量qRT-PCR和Westernblot检测不同实验组膀胱癌细胞中miR-200c、KRAS的表达量,克隆试验检测不同组膀胱癌细胞系的增殖能力。结果CCK-8试验显示对照组T24和UM-UC-3与miR-200c组分别为0.8333±0.0621、0.6503±0.0887和0.9390±0.0181、0.8060±0.0216,2组间差异有统计学意义(T24,P<0.05;UM-UC-3,P<0.01)。稳定过表达miR-200c后,膀胱癌细胞系内KRAS蛋白的表达降低(P<0.05)。结论miR-200c在体外通过下调KRAS基因降低膀胱癌细胞的增殖能力。

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