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调节软骨细胞骨架蛋白对成软骨表型的影响

, PP. 2137-2141

Keywords: 软骨细胞,细胞骨架,微团培养,软骨表型,骨关节炎

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Abstract:

目的研究软骨细胞骨架蛋白结构改变与成软骨细胞表型改变的关系。方法采用三维微团培养法培养新西兰兔膝关节软骨细胞,将软骨细胞分为对照组、秋水仙碱组、细胞松弛素B组及丙烯酰胺组,通过秋水仙碱、细胞松弛素B及丙烯酰胺分别调节软骨细胞骨架的微管、肌动蛋白微丝及波形蛋白中间纤丝,从大体形态、组织切片染色、免疫组化染色及RT-PCR分析成软骨细胞表型的改变。结果对照组、秋水仙碱组及细胞松弛素B组细胞较密集、均一,丙烯酰胺组细胞较少、分散。对照组、秋水仙碱组及细胞松弛素B组COLⅡ、蛋白多糖染色深,COLⅠ染色浅;而丙烯酰胺组COLⅡ、蛋白多糖染色浅,COLⅠ染色较深。RT-PCR数据统计分析显示对照组与秋水仙碱组、细胞松弛素B组相比,COLⅠ(1.21±0.21vs1.55±0.06、1.70±0.07)及COLⅡ(1.10±0.12vs0.91±0.21、0.90±0.30)表达差异无统计学意义(P>0.05);而对照组COLⅡ表达明显多于丙烯酰胺组(0.26±0.06),COLⅠ表达明显低于丙烯酰胺组(9.63±0.38),差异有统计学意义(P<0.05)。结论丙烯酰胺调节软骨细胞骨架波形蛋白中间纤丝可明显下调COLⅡ、蛋白多糖的表达。

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